Capturing individual charged microparticles and cells with microelectrodes in a circular microfluidic channel

Capture and, subsequently, in situ characterization of microparticles generally require complex fabrication processes and sophisticated techniques microfluidic devices. In this paper, we present a simple inexpensive technique for capturing applying chemical agents to individual charged with the same glass microelectrode circular channel. The device features thin poly-dimethyl-siloxane (PDMS) layer (100 µm, thickness) above channel (diameter, 50 µm) so that tip can enter into by penetrating through PDMS layer. When an electric field is applied across microelectrodes, capture or living cells are flowing Meanwhile, be used apply captured particles filling solution agent its tip. This design method offered without need any specialized equipment manipulation controlled microenvironments.